Non-Visual Opsin 3 Localizes with Microtubules During Mitosis and is Essential for Cytokinesis

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F. Labarrade, C. Gondran, K. Cucumel

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Published: 8 January 2019 | Article Type :

Abstract

Opsin photoreceptors are responsible for the absorption of light, transduce information about daily lighting conditions and provide vision. Opsins are also involved in diverse non-visual functions such as the circadian clock. The non-visual opsin 3, also known as encephalopsin, is expressed in skin and its functions remain poorly defined.
The expression of opsin 3 in human skin tissue was evaluated by immunohistochemistry, in keratinocytes and melanocytes by immunodetection. Opsin 3 and β-tubulin colocalization was performed during the keratinocyte mitotic phase. Opsin 3-siRNA transfection was used in primary keratinocytes and successful transfection was confirmed by immunodetection.
These results show that opsin 3 was detected in the epidermis of human skin, both in keratinocytes and melanocytes. Opsin 3 colocalize with β-tubulin during the mitotic phase and participate in keratinocyte cell division. Opsin 3 protein expression was reduced after transfection with opsin 3-siRNA; inhibition of opsin 3-siRNA reduces keratinocyte cell proliferation and promotes the apparition of binucleated cells.
This study highlights an unconventional function of opsin 3 in extra-ocular cells. Opsin 3 appears to be involved in the regulation of late cytokinesis in keratinocytes, and therefore in the maintenance of epidermal homeostasis.

Keywords: Skin; Keratinocyte; Melanocyte; Photoreceptors; Opsin 3; Mitosis; Cytokinesis.

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F. Labarrade, C. Gondran, K. Cucumel. (2019-01-08). "Non-Visual Opsin 3 Localizes with Microtubules During Mitosis and is Essential for Cytokinesis." *Volume 2*, 1, 1-7